CENS Medium

[所属分类:培养基配方] [发布时间:2020-4-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CENS Medium

培养基配方
Composition per liter:
Papaic digest of soybean meal....................................4.0g
NH4Cl ...........................................................1.0g
K2HPO4 ..........................................................0.9g
KH2PO4 ..........................................................0.6g
Na2S2O3 ·5H2O...................................................0.5g
MgSO4 ·7H2O.....................................................0.2g
CaCl2 ·2H2O...................................................75.0mg
EDTA............................................................5.0mg
Vitamin B 12 .................................................20.0μg
D -Biotin.....................................................15.0μg
Sodium pyruvate solution.......................................10.0mL
Chelated iron solution..........................................2.0mL
True Blue trace elements........................................1.0mL
pH 6.8 ± 0.2 at 25°C
Sodium Pyruvate Solution:
Composition per 10.0mL:
Sodium pyruvate..................................................2.2g
Preparation of Sodium Pyruvate Solution:  Add sodium pyru-
vate to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Chelated Iron Solution:
Composition per 900.0mL:
EDTA......................................................2.0g
FeCl2 ·4H2O..............................................1.0g
HCl, concentrated .......................................3.0mL
Preparation of Chelated Iron Solution: Add components to dis-
tilled/deionized water and bring volume to 900.0mL. Mix thoroughly.
True Blue Trace Elements:
Composition per 250.0mL:
EDTA.....................................................2.5g
MnCl2 . 4H2O.............................................0.2g
H3BO3 ...................................................0.1g
Na2MoO4 ·2H2O ..........................................0.1g
ZnCl2..................................................50.0mg
NiCl2 ·6H2O...........................................50.0mg
CoCl2 ·6H2O...........................................20.0mg
CuCl2 ·2H2O...........................................10.0mg
Na2SeO3 ................................................5.0mg
NaVO3 ..................................................5.0mg
Preparation of True Blue Trace Elements: Add components,
one at a time, to 200.0mL of distilled/deionized water. Mix thoroughly.
Bring volume to 250.0mL with distilled/deionized water.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation of Rhodospirillum centenum.