CF Assay Medium (Citrovorum Factor Assay Medium)

[所属分类:培养基配方] [发布时间:2020-4-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CF Assay Medium
(Citrovorum Factor Assay Medium)

培养基配方

Composition per liter:
Glucose ........................................................50.0g
Sodium acetate..................................................40.0g
Vitamin assay casamino acids....................................10.0g
NH4Cl ...........................................................6.0g
K2HPO4 ..........................................................1.2g
KH2PO4 ..........................................................1.2g
MgSO4 ·7H2O.....................................................0.4g
DL- Alanine......................................................0.2g
DL- Tryptophan...................................................0.2g
L- Cystine ......................................................0.2g
L- Cysteine·HCl.................................................0.2g
MgSO4 ·7H2O....................................................0.04g
Adenine sulfate.................................................0.02g
FeSO4 ..........................................................0.02g
Glycine.........................................................0.02g
Guanine·HCl....................................................0.02g
NaCl............................................................0.02g
Uracil .........................................................0.02g
Xanthine........................................................0.02g
Pyridoxamine·HCl ..............................................6.0mg
Nicotinic acid..................................................2.0mg
Pyridoxine·HCl.................................................2.0mg
Calcium pantothenate ...........................................1.0mg
Riboflavin .....................................................1.0mg
Thiamine·HCl ..................................................1.0mg
Pyridoxal·HCl...............................................600.0μg
p-Aminobenzoic acid..........................................200.0μg
Folic acid....................................................20.0μg
Biotin ........................................................2.0μg
pH 6.7 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Continue boiling for 2–3 min. Allow precipitate to settle out.
Distribute supernatant into tubes in 5.0mL volumes. Add standard so-
lution or test solutions to each tube. Adjust the volume of each tube to
10.0mL with distilled/deionized water. Autoclave for 10 min at 15 psi
pressure–121°C.
Use: For the microbiological assay of citrovorum factor using Pedio-
coccus acidilactici.