CFAT Medium

[所属分类:培养基配方] [发布时间:2020-4-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CFAT Medium
(Cadmium Fluoride Acriflavin Tellurite Medium)

培养基配方
Composition per liter:
Pancreatic digest of casein....................................17.0g
Agar...........................................................15.0g
Glucose.........................................................7.5g
NaCl............................................................5.0g
Papaic digest of soybean meal...................................3.0g
K2HPO4 .........................................................2.5g
NaF ............................................................0.8g
CdSO4 ........................................................0.013g
K2TeO3 ........................................................2.5mg
Neutral acriflavin.............................................1.2mg
Basic Fuchsin.................................................0.25mg
Sheep blood, defibrinated.....................................50.0mL
Caution: Potassium tellurite is toxic.
Preparation of Medium: Add components, except sheep blood, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Add 50.0mL of sterile, defibrinat-
ed sheep blood. Mix thoroughly. Pour into sterile Petri dishes or leave
in tubes.
Use: For the isolation, cultivation, and enumeration of Actinomyces
viscosus and Actinomyces naeslundii from clinical specimens, espe-
cially dental plaque.