Chalquist’s Antigen Medium, Modified

[所属分类:培养基配方] [发布时间:2020-4-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chalquist’s Antigen Medium, Modified

培养基配方
Composition per liter:
Soluble starch..............................................0.5g
Pancreatic digest of casein................................0.05g
L -Cysteine·HCl·H2O .....................................0.01g
NAD (nicotinamide adeninedinucleotide).....................0.01g
PPLO broth without Crystal Violet.........................90.0mL
Swine serum, inactivated..................................10.0mL
Phenol Red (1% solution)..................................0.25mL
pH 7.6 ± 0.2 at 25°C
PPLO Broth without Crystal Violet:
Composition per 500.0mL:
Beef heart, infusion from.................................11.52g
Peptone....................................................2.32g
NaCl.......................................................1.15g
Source: PPLO broth without Crystal Violet is available as a premixed
powder from BD Diagnostic Systems.
Preparation of PPLO Broth without Crystal Violet:  Add
components to distilled/deionized water and bring volume to 500.0mL.
Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation of Mycoplasma synoviae.