Charcoal Agar Base, HiVeg with Blood and Selective Supplement

[所属分类:培养基配方] [发布时间:2020-4-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Charcoal Agar Base, HiVeg
with Blood and Selective Supplement

培养基配方
Composition per liter:
Agar ...........................................................18.0g
Plant infusion..................................................12.0g
Plant peptone...................................................10.0g
Starch .........................................................10.0g
NaCl.............................................................5.0g
Charcoal.........................................................4.0g
Yeast extract....................................................3.5g
Sheep blood, defibrinated......................................50.0mL
Selective supplement solution .................................10.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium, without blood or selective supplement, is
available as a premixed powder from HiMedia.
Selective Supplement Solution:
Composition per 10.0mL:
Cefazolin......................................................40.0mg
Preparation of Selective Supplement Solution: Add cefazolin
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except blood and se-
lective supplement solution, to distilled/deionized water and bring vol-
ume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling with
frequent stirring. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Aseptically add 50.0 mL sterile sheep blood and 10.0mL
sterile selective supplement solution. Mix thoroughly. Pour into sterile
Petri plates or distribute into sterile tubes.
Use: For the cultivation and isolation of various bacteria; with the
addition of blood, for the cultivation of fastidious bacteria. For the cul-
tivation of Bordetella pertussis for vaccine production and the mainte-
nance of stock cultures.