Charcoal Agar with Horse Blood and Cepahalexin

[所属分类:培养基配方] [发布时间:2020-4-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Charcoal Agar with Horse Blood and Cepahalexin

培养基配方
Composition per liter:
Agar............................................................12.0g
Beef extract....................................................10.0g
Peptone ........................................................10.0g
Starch..........................................................10.0g
NaCl.............................................................5.0g
Charcoal.........................................................4.0g
Nicotinic acid..................................................1.0mg
Horse blood, defibrinated ....................................100.0mL
Cephalexin solution............................................10.0mL
pH 7.4 ± 0.2 at 25°C
Cephalexin Solution:
Composition per 10.0mL:
Cephalexin......................................................0.04g
Preparation of Cephalexin Solution: Add cephalexin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except cephalexin so-
lution and horse blood, to distilled/deionized water and bring volume
to 890.0L. Mix thoroughly. Gently heat and bring to boiling with fre-
quent stirring. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically add 100.0mL of sterile, defibrinated horse
blood and 10.0mL of sterile cephalexin solution. Pour into sterile Petri
dishes or distribute into tubes. Shake flask while dispensing to keep
charcoal in suspension.
Use: For the cultivation and isolation of Bordetella pertussis.