Charcoal Blood Agar Base, HiVeg with Blood Penicillin and Damidodiphenyl Hydrochloride

[所属分类:培养基配方] [发布时间:2020-4-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Charcoal Blood Agar Base, HiVeg with Blood Penicillin
and Damidodiphenyl Hydrochloride

培养基配方
Composition per liter:
Agar............................................................12.0g
Plant extract...................................................10.0g
Plant peptone ..................................................10.0g
Starch..........................................................10.0g
NaCl.............................................................5.0g
Charcoal.........................................................4.0g
Yeast extract....................................................3.5g
Sheep blood, defibrinated......................................50.0mL
Penicillin solution.............................................3.0mL
Diamidodiphenylamine hydrochloride solution.....................3.0mL
pH 7.5 ± 0.2 at 25°C
Source: This medium, without blood or selective supplement, is
available as a premixed powder from HiMedia.
Penicllin Solution:
Composition per 10.0mL:
Penicillin...................................................1000 units
Preparation of Penicillin Solution: Add penicillin to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Diamidodiphenylamine Hydrochloride Solution:
Composition per 10.0mL:
Diamido hydrochloride..........................................0.01g
Diphenylamine hydrochloride ...................................0.01g
Preparation of Diamidodiphenylamine Hydrochloride So-
lution: Add components to distilled/deionized water and bring vol-
ume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except blood, penicil-
lin solution, and diamidodiphenylamine hydrochloride solution, to dis-
tilled/deionized water and bring volume to 950.0mL. Mix thoroughly.
Gently heat and bring to boiling with frequent stirring. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add
50.0 mL sterile sheep blood and 3.0 mL sterile penicillin solution and
3.0 mL sterile diamidodiphenylamine hydrochloride solution. Mix
thoroughly. Pour into sterile Petri plates or distribute into sterile tubes.
Use: For the cultivation and isolation of various bacteria; with the
addition of blood, for the cultivation of fastidious bacteria. For the cul-
tivation of Bordetella pertussis for vaccine production and the mainte-
nance of stock cultures.