Charcoal Blood Medium

[所属分类:培养基配方] [发布时间:2020-4-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Charcoal Blood Medium

培养基配方
Composition per liter:
Beef heart, solids from infusion..............................500.0g
Agar ..........................................................18.0g
Peptone........................................................10.0g
Soluble starch.................................................10.0g
NaCl............................................................5.0g
Charcoal, activated, acid washed................................4.0g
Yeast extract...................................................3.5g
Horse or sheep blood, defibrinated...........................100.0mL
Cephalexin solution ..........................................10.0mL
pH 7.4 ± 0.2 at 25°C
Cephalexin Solution:
Composition per 10.0mL:
Cephalexin....................................................0.04g
Preparation of Cephalexin Solution: Add cephalexin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except blood and ce-
phalexin solution, to distilled/deionized water and bring volume to
890.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add sterile blood and cephalexin solution. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Haemophilus influenzae.