Charcoal HiVeg Agar Base with Niacin, Blood, and Selective Supplement

[所属分类:培养基配方] [发布时间:2020-4-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Charcoal HiVeg Agar Base
with Niacin, Blood, and Selective Supplement

培养基配方

Composition per liter:
Agar ..........................................................12.0g
Plant extract .................................................10.0g
Plant peptone No.2.............................................10.0g
Starch ........................................................10.0g
NaCl............................................................5.0g
Charcoal........................................................4.0g
Niacin.........................................................1.0mg
Sheep blood, defibrinated.....................................50.0mL
Selective supplement solution ................................10.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium, without blood or selective supplement, is
available as a premixed powder from HiMedia.
Selective Supplement Solution:
Composition per 10.0mL:
Cefazolin......................................................40.0mg
Preparation of Selective Supplement Solution: Add cefazolin
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except blood and se-
lective supplement solution, to distilled/deionized water and bring vol-
ume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling with
frequent stirring. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Aseptically add 50.0 mL sterile sheep blood and 10.0mL
sterile selective supplement solution. Mix thoroughly. Pour into sterile
Petri plates or distribute into sterile tubes.
Use: For the cultivation and isolation of Bordetella pertussis and Hae-
mophilus influenzae. For the cultivation of Bordetella pertussis for
vaccine production and the maintenance of stock cultures.