Chase’s Medium SP

[所属分类:培养基配方] [发布时间:2020-4-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chase’s Medium SP

培养基配方
Composition per liter:
Agar.......................................................10.0g
Proteose peptone...........................................10.0g
K2HPO4 .....................................................2.0g
(NH4)2SO4 ..................................................1.0g
Sucrose solution.........................................100.0mL
pH 6.5 ± 0.2 at 25°C
Sucrose Solution:
Composition per 100.0mL:
Sucrose...................................................10.0g
Preparation of Sucrose Solution: Add sucrose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium:  Add components, except sucrose solu-
tion, to tap water and bring volume to 900.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL of sterile
sucrose solution. Mix thoroughly. Pour into sterile Petri dishes or dis-
tribute into sterile tubes.
Use:  For the cultivation and maintenance of ATCC strain 13949.