CHCA Salts Medium (Cyclohexane Carboxylic Acid Salts Medium)

[所属分类:培养基配方] [发布时间:2020-4-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CHCA Salts Medium
(Cyclohexane Carboxylic Acid Salts Medium)

培养基配方
Composition per liter:
K2HPO4 ........................................................3.5g
KH2PO4 ........................................................1.5g
Cyclohexane carboxylic acid....................................1.0g
NH4NO3 ........................................................1.0g
MgSO4 ·7H2O...................................................0.5g
FeSO4 ·7H2O...................................................0.1g
Yeast extract..................................................0.1g
CaCl2 ·2H2O..................................................0.01g
Na2MoO2 ·2H2O ...............................................0.01g
ZnSO4 ·7H2O..................................................0.01g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes.
Use:  For the cultivation and maintenance of bacteria that can utilize
cyclohexane carboxylic acid as a carbon source. For the cultivation and
maintenance of Arthrobacter globiformis.