China Blue Lactose Agar

[所属分类:培养基配方] [发布时间:2020-4-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

China Blue Lactose Agar

培养基配方
Composition per liter:
Agar ..........................................................12.0g
Lactose........................................................10.0g
Peptone.........................................................5.0g
NaCl............................................................5.0g
Beef extract....................................................3.0g
China Blue ...................................................0.375g
pH 7.0 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Oxoid
Unipath.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation, differentiation, and enumeration of bacteria
from dairy products. Lactose-fermenting bacteria appear as blue colo-
nies. Nonlactose-fermenting bacteria appear as colorless colonies.