Chlorella Broth

[所属分类:培养基配方] [发布时间:2020-4-20] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chlorella Broth

培养基配方
Composition per liter:
KNO3 ...........................................................2.5g
KH2PO4 ........................................................2.45g
MgSO4 ·7H2O....................................................2.4g
K2SO4 ........................................................0.217g
FeSO4 ·7H2O...................................................1.5mg
MnSO4 ·H2O....................................................1.4mg
H3BO3 ........................................................0.28mg
ZnSO4 ·7H2O..................................................0.22mg
Na2MoO4 ·2H2O................................................0.05mg
CuSO4 ·5H2O................................................0.0078mg
Supplement solution..........................................100.0mL
pH 4.5 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Supplement Solution:
Composition per 100.0mL:
Glucose......................................................10.0g
Potassium citrate...........................................32.0mg
Preparation of Supplement Solution:  Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except supplement so-
lution, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°C. Aseptically add supplement solution. Mix thoroughly. Asepti-
cally distribute into sterile tubes.
Use: For the cultivation of Chlorella spp.