Chlorinated Fatty Acid Medium

[所属分类:培养基配方] [发布时间:2020-4-20] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chlorinated Fatty Acid Medium

培养基配方
Composition per 1001.0mL:
(NH4)2SO4 .................................................0.5g
MgSO4 ·7H2O...............................................0.1g
Ca(NO3)2 ................................................0.075g
2-Chloropropionate........................................0.54g

Phosphate buffer (20mM solution, pH 7.2)...................1.0L
Trace elements solution ..................................1.0mL
pH 7.2 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
FeSO4 ·7H2O...............................................1.0g
MnSO4 ·H2O................................................1.0g
Co(NO3)2 ·6H2O ..........................................0.25g
CuCl2 ·2H2O .............................................0.25g
Na2MoO4 ·2H2O ...........................................0.25g
ZnCl2 ....................................................0.25g
H3BO3 .....................................................0.1g
NH4VO3 ....................................................0.1g
NiSO4 ·6H2O...............................................0.1g
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Combine components. Mix thoroughly.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation of Alcaligenes species.