Chlorobiaceae Medium 2

[所属分类:培养基配方] [发布时间:2020-4-20] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chlorobiaceae Medium 2

培养基配方
Composition per 1051.0mL:
Solution 1...................................................950.0mL
Na2S·9H2O solution...........................................60.0mL
NaHCO3 solution...............................................40.0mL
Vitamin B 12 solution..........................................1.0mL
pH 6.8 ± 0.2 at 25°C
Solution 1:
Composition per 950.0mL:
KH2PO4 .........................................................1.0g
NH4Cl ..........................................................0.5g
MgSO4 ·7H2O....................................................0.4g
CaCl2 ·2H2O ..................................................0.05g
Trace elements solution SL-8...................................1.0mL
Preparation of Solution 1:  Add components to distilled/deionized
water and bring volume to 950.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C.
Trace Elements Solution SL-8:
Composition per liter:
Disodium EDTA ..................................................5.2g
FeCl2 ·4H2O....................................................1.5g
CoCl2 ·6H2O ..................................................0.19g
MnCl2 ·4H2O....................................................0.1g
ZnCl2 .........................................................0.07g
H3BO3 .........................................................0.06g
NaMoO4 ·2H2O..................................................0.04g
CuCl2 ·2H2O ..................................................0.02g
NiCl2 ·6H20...................................................0.02g
Preparation of Trace Elements Solution SL-8: Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly.
Na2S·9H2O Solution:
Composition per 100.0mL:
Na2S·9H2O.......................................................5.0g
Preparation of Na2S·9H2O Solution:  Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 100.0mL. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C.
NaHCO3 Solution:
Composition per 100.0mL:
NaHCO3 ..........................................................5.0g
Preparation of NaHCO3 Solution:  Add NaHCO3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Vitamin B 12 Solution:
Composition per 100.0mL:
Vitamin B 12 ...................................................2.0mg
Preparation of Vitamin B 12 Solution:  Add vitamin B 12 to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium:  To 950.0mL of cooled, sterile solution 1,
aseptically add 60.0mL of sterile Na2S·9H2O solution, 40.0mL of ster-
ile NaHCO3 solution, and 1.0mL of sterile vitamin B 12 solution. Mix
thoroughly. Adjust pH to 6.8 with sterile H2SO4 or Na2CO3 . Aseptical-
ly distribute into sterile 50.0mL or 100.0mL bottles with metal screw-
caps and rubber seals. Completely fill bottles with medium except for
a pea-sized air bubble.
Use: For the isolation and cultivation of freshwater and soil members
of the Chlorobiaceae.