Chlorobium thiosulfatophilum Medium

[所属分类:培养基配方] [发布时间:2020-4-20] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chlorobium thiosulfatophilum Medium

培养基配方
Composition per 1050.0mL:
KH2PO4 .....................................................1.0g
NH4Cl ......................................................1.0g
MgCl2 ·6H2O................................................0.5g
Solution A................................................20.0mL
Solution B................................................20.0mL
Solution C................................................10.0mL
Trace elements solution ...................................1.0mL
pH 7.0 ± 0.2 at 25°C
Solution A:
Composition per 100.0mL:
NaHCO3 .....................................................10.0g
Preparation of Solution A:  Add NaHCO3 to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C.
Solution B:
Composition per 100.0mL:
Na2S·9H2O....................................................10.0g
Preparation of Solution B:  Add Na2S·9H2O to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C.
Solution C:
Composition per 100.0mL:
Na2S2O3 ·9H2O.................................................10.0g
Preparation of Solution C:  Add Na2S2O3 ·9H2O to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C.
Trace Elements Solution:
Composition per liter:
FeCl3 ·6H2O...................................................2.7g
H3BO3 .........................................................0.1g
ZnSO4 ·7H2O...................................................0.1g
Co(NO3)2 ·6H2O..............................................50.0mg
CuSO4 ·5H2O..................................................5.0mg
MnCl2 ·6H2O .................................................5.0mg
Preparation of Trace Elements Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except solution A, so-
lution B, and solution C, to distilled/deionized water and bring volume
to 1.0L. Mix thoroughly. Bring pH to 7.0–7.2 with H3PO4 . Distribute
into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Aseptically add 0.2mL of sterile solution A, 0.2mL of sterile solution
B, and 0.1mL of sterile solution C for each 10.0mL of medium. Mix
thoroughly. Use immediately.
Use: For the cultivation and maintenance of Chlorobium limnicola.