Chloroflexus Agar

[所属分类:培养基配方] [发布时间:2020-4-20] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chloroflexus Agar

培养基配方
Composition per liter:
Agar...........................................................15.0g
Glycyl-glycine .................................................0.5g
Yeast extract...................................................0.5g
Na2S............................................................0.5g
NH4Cl ..........................................................0.2g
MgSO4 ·7H2O....................................................0.1g
Nitrilotriacetic acid...........................................0.1g
NaNO3 ........................................................0.689g
Na2HPO4 ......................................................0.111g
KNO3 .........................................................0.103g
CaSO4 ·2H2O...................................................0.06g
NaCl...........................................................8.0mg
FeCl3 solution ................................................1.0mL
Micronutrient solution ........................................1.0mL
pH 8.2–8.4 at 25°C
FeCl3 Solution:
Composition per liter:
FeCl3 ..........................................................0.29g
Preparation of FeCl3 Solution: Add FeCl3 to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly.
Micronutrient Solution:
Composition per liter:
MnSO4 ·7H2O.....................................................2.28g
H3BO3 ............................................................0.5g
ZnSO4 ·7H2O......................................................0.5g
CoCl2 ·6H2O ...................................................0.045g
CuSO4 ·2H2O....................................................0.025g
Na2MoO4 ·2H2O .................................................0.025g
H2SO4 , concentrated.............................................0.5mL
Preparation of Micronutrient Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except Na2S, to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad-
just pH to 8.2–8.4. Add Na2S. Readjust pH to 8.2–8.4. Gently heat and
bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in
tubes.
Use:  For the cultivation of Chloroflexus aurantiacus.