CHO HiVeg Medium Base with Carbohydrate Solution

[所属分类:培养基配方] [发布时间:2020-4-21] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CHO HiVeg Medium Base
with Carbohydrate Solution

培养基配方
Composition per liter:
Plant hydrolysate .......................................15.0g
Yeast extract.............................................7.0g
NaCl......................................................2.5g
Agar.....................................................0.75g
Na-thioglycollate.........................................0.5g
L -Cystine...............................................0.25
Ascorbic acid.............................................0.1g
Bromthymol Blue..........................................0.01g
Carbohydrate soltuion...................................6.25mL
pH 7.0 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Carbohydrate Solution:
Composition per 100.0mL:
Carbohydrate.............................................10.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 100.0mL. Adonitol, ara-
binose, cellobiose, glucose, dulcitol, fructose, galactose, inositol, lac-
tose, maltose, mannitol, raffinose, rhamnose, salicin, sorbitol, sucrose,
trehalose, xylose, or other carbohydrates may be used. Mix thoroughly.
Filter sterilize.
Preparation of Medium:  Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat and bring to boiling. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Aseptically add 6.25mL of sterile carbohydrate solution. Aseptically
distribute into sterile tubes or leave in flasks.
Use: Used as a basal medium to which carbohydrates are added for
fermentation studies of anaerobic bacteria.