Chlorohydroxybenzoic Acid Medium

[所属分类:培养基配方] [发布时间:2020-4-21] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chlorohydroxybenzoic Acid Medium

培养基配方
Composition per liter:
K2HPO4 ·3H2O .............................................4.25g
NH4Cl ......................................................2.0g
NaH2PO4 ·H2O...............................................1.0g
5-Chloro-2-hydroxybenzoic acid..............................0.5g
MgSO4 ·7H2O................................................0.2g
Nitrilotriacetic acid.......................................0.1g
FeSO4 ·7H2O..............................................0.012g
MnSO4 ·H2O................................................3.0mg
ZnSO4 ·7H2O...............................................3.0mg
CoSO4 .....................................................1.0mg
pH 7.0-7.4 at 25°C
Preparation of Medium:  Add 5-chloro-2-hydroxybenzoic acid to
800.0mL of distilled/deionized water. Adjust pH to 7.0 with NaOH.
Add remaining components and bring volume to 1.0L. Distribute into
tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation of bacteria that can utilize 5-chloro-hydroxy-
benzoic acid. For the cultivation of ATCC strain 35944.