Chloroflexus Medium, Modified

[所属分类:培养基配方] [发布时间:2020-4-21] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chloroflexus Medium, Modified

培养基配方
Composition per 1001.0 mL:
Glycyl-glycine .............................................1.0g
Yeast extract...............................................1.0g
NaNO3 ......................................................0.5g
KNO3 .......................................................0.1g
MgSO4 ·7H2O................................................0.1g
Na2HPO4 ·2H2O..............................................0.1g
NaCl........................................................0.1g
CaCl2 ·2H2O...............................................0.05g
Neutralized sulfide solution..............................11.0mL
Ferric citrate solution....................................1.0mL
Trace elements solution SL-6 ..............................1.0mL
pH 8.2 ± 0.2 at 25°C
Neutralized Sulfide Solution:
Composition per 100.0mL:
Na2S·9H2O..................................................1.5g
Preparation of Neutralized Sulfide Solution : Add Na2S·9H2O
to distilled/deionized water in a 250mL screw-capped bottle fitted with
a butyl rubber septum and bring volume to 100.0mL. Add a magnetic
stir bar. Mix thoroughly. Sparge under 100% N2 gas for 3 min. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to room temperature.
Adjust pH to about 7.3 with sterile 2MH2SO  . Do not open the bottle
to add H2SO4 ; use a sterile syringe. Stir the solution continuously to
avoid precipitation of elemental sulfur. The final solution should be
clear and yellow in color.
Ferric Citrate Solution:
Composition per 100.0mL:
Ferric citrate..............................................0.1g
Preparation of Ferric Citrate Solution: Add ferric citrate to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Trace Elements Solution SL-6:
Composition per liter:
MnCl2 ·4H2O.....................................................0.5g
H3BO3 ...........................................................0.3g
CoCl2 ·6H2O ....................................................0.2g
ZnSO4 ·7H2O.....................................................0.1g
Na2MoO4 ·2H2O .................................................0.03g
NiCl2 ·6H2O....................................................0.02g
CuCl2 ·2H2O ...................................................0.01g
Preparation of Trace Elements Solution SL-6:  Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except neutralized sul-
fide solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Gently heat and bring to boiling. Continue
boiling for 3–4 min under 100% N2 . Distribute 90.0mL of medium into
100mL screw-capped bottles under 100% N2 . Autoclave for 15 min at
15 psi pressure–121°C. Cool to room temperature. Using a sterile sy-
ringe, inject 1.0mL of neutralized sulfide solution into each bottle.
Use: For the growth and maintenance of Chloroflexus aurantiacus.