Chloroflexus Broth

[所属分类:培养基配方] [发布时间:2020-4-21] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chloroflexus Broth

培养基配方
Composition per liter:
NaNO3 ....................................................0.689g
Glycyl-glycine .............................................0.5g
Yeast extract...............................................0.5g
Na2S........................................................0.5g
NH4Cl ......................................................0.2g
Na2HPO4 ..................................................0.111g
KNO3 .....................................................0.103g
MgSO4 ·7H2O................................................0.1g
Nitrilotriacetic acid.......................................0.1g
CaSO4 ·2H2O...............................................0.06g
NaCl.......................................................8.0mg
FeCl3 solution.............................................1.0mL
Micronutrient solution.....................................1.0mL
pH 8.2–8.4 at 25°C
FeCl3 Solution:
Composition per liter:
FeCl3 .....................................................0.29g
Preparation of FeCl3 Solution: Add FeCl3 to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly.
Micronutrient Solution:
Composition per liter:
MnSO4 ·7H2O...............................................2.28g
H3BO3 ......................................................0.5g
ZnSO4 ·7H2O................................................0.5g
CoCl2 ·6H2O .............................................0.045g
CuSO4 ·2H2O..............................................0.025g
Na2MoO4 ·2H2O ...........................................0.025g
H2SO4 , concentrated.......................................0.5mL
Preparation of Micronutrient Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except Na2S, to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad-
just pH to 8.2–8.4. Add Na2S. Readjust pH to 8.2–8.4. Filter sterilize.
Distribute into sterile tubes or flasks.
Use:  For the cultivation of Chloroflexus aurantiacus.