Chocolate Agar

[所属分类:培养基配方] [发布时间:2020-4-21] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chocolate Agar

培养基配方
Composition per liter:
Agar .......................................................15.0g
Pantone.....................................................10.0g
Bitone......................................................10.0g
NaCl.........................................................5.0g
Tryptic digest of beef heart.................................3.0g
Cornstarch...................................................1.0g
Sheep blood, defibrinated.................................100.0mL
Supplement B...............................................10.0mL
pH 7.3 ± 0.2 at 25°C
Supplement B:
Composition per 10.0mL:
Cephalothin................................................15.0mg
Vancomycin ................................................10.0mg
Trimethoprim................................................5.0mg
Amphotericin B..............................................2.0mg
Polymyxin B.................................................2500U
Preparation of Supplement B:  Add components to 10.0mL of dis-
tilled/deionized water. Mix thoroughly. Filter sterilize.
Source:  Supplement B is available from BD Diagnostic Systems.
Preparation of Medium: Add components, except supplement B
solution and sheep blood, to distilled/deionized water and bring vol-
ume to 890.0mL. Mix thoroughly. Gently heat until boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add 100.0mL of sterile, defibrinated sheep blood. Gently heat while
stirring and bring to 85°C for 5–10 min. Cool to 50°C. Aseptically add
10.0mL of sterile supplement B. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of a variety of fastidious micro-
organisms.