Chocolate Agar

[所属分类:培养基配方] [发布时间:2020-4-21] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chocolate Agar

培养基配方
Composition per liter:
Proteose peptone No.3......................................15.0g
Agar.......................................................10.0g
NaCl........................................................5.0g
K2HPO4 .....................................................4.0g
Cornstarch..................................................1.0g
KH2PO4 .....................................................1.0g
Hemoglobin solution .....................................100.0mL
Supplement B..............................................10.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium is available from BD Diagnostic Systems.
Supplement B:
Composition per 10.0mL:
Cephalothin...............................................15.0mg
Vancomycin................................................10.0mg
Trimethoprim...............................................5.0mg
Amphotericin B ............................................2.0mg
Polymyxin B................................................2500U
Preparation of Supplement B:  Add components to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Hemoglobin Solution:
Composition per 100.0mL:
Hemoglobin.................................................10.0g
Preparation of Hemoglobin Solution: Add hemoglobin to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except hemoglobin so-
lution and supplement B, to distilled/deionized water and bring volume
to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti-
cally add 100.0mL of sterile hemoglobin solution. Gently heat while
stirring and bring to 85°C for 5–10 min. Cool to 50°C. Aseptically add
10.0mL of sterile supplement B. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of fastidious microorganisms.