Chocolate Agar, Enriched

[所属分类:培养基配方] [发布时间:2020-4-21] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chocolate Agar, Enriched

培养基配方
Composition per liter:
GC medium base..................................................740.0mL
Hemoglobin solution ............................................250.0mL
Supplement B.....................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium is available from BD Diagnostic Systems.
GC Medium Base:
Composition per 740.0mL:
Agar...............................................................20.0g
Proteose peptone No.3..............................................15.0g
NaCl................................................................5.0g
K2HPO4 .............................................................4.0g
Glucose.............................................................1.5g
Cornstarch..........................................................1.0g
KH2PO4 .............................................................1.0g
pH 7.2 ± 0.2 at 25°C
Preparation of GC Medium Base: Add components to distilled/
deionized water and bring volume to 740.0mL. Mix thoroughly. Gently
heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Hemoglobin Solution:
Composition per 250.0mL:
Hemoglobin .......................................................10.0g
Preparation of Hemoglobin Solution: Add hemoglobin to dis-
tilled/deionized water and bring volume to 250.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Supplement B:
Composition per 10.0mL:
Cephalothin...................................................15.0mg
Vancomycin ...................................................10.0mg
Trimethoprim...................................................5.0mg
Amphotericin B.................................................2.0mg
Polymyxin B....................................................2500U
Preparation of Supplement B:  Add components to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: To 740.0mL of cooled sterile GC medium
base, aseptically add 250.0mL of sterile hemoglobin solution and
10.0mL of sterile supplement B. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the cultivation of fastidious microorganisms, especially Neis-
seria species.