Chocolate Agar-Bartonella C-29 (ATCC Medium 2119)

[所属分类:培养基配方] [发布时间:2020-4-21] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chocolate Agar-Bartonella C-29
(ATCC Medium 2119)

培养基配方
Composition per 1010.0mL:
GC agar base solution.........................................500.0 ml
Hemoglobin solution ..........................................500.0 ml
IsoVitaleX ® enrichment.........................................10.0mL
IsoVitaleX ® Enrichment:
Composition per liter:
Glucose.........................................................100.0g
L- Cysteine·HCl.................................................25.9g
L- Glutamine ....................................................10.0g
L- Cystine........................................................1.1g
Adenine...........................................................1.0g
Nicotinamide adenine dinucleotide ...............................0.25g
Vitamin B 12 .....................................................0.1g
Thiamine pyrophosphate ...........................................0.1g
Guanine·HCl.....................................................0.03g
Fe(NO3)3 ·6H2O..................................................0.02g
p-Aminobenzoic acid.............................................0.013g
Thiamine·HCl....................................................3.0mg
Preparation of IsoVitaleX ® : Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.
GC Agar Base Solution:
Composition per 500.0mL:
Agar.............................................................10.0g
Pancreatic digest of casein.......................................7.5g
Peptic digest of animal tissue ...................................7.5g
NaCl..............................................................5.0g
K2HPO4 ...........................................................4.0g
Cornstarch........................................................1.0g
KH2PO4 ...........................................................1.0g
Preparation of GC Agar Base: Add components to distilled/deion-
ized water and bring volume to 500.0mL. Mix thoroughly. Gently heat
until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C.
Hemoglobin Solution:
Composition per 500.0mL:
Hemoglobin.....................................................10.0g
Preparation of Hemoglobin Solution: Add hemoglobin to dis-
tilled/deionized water and bring volume to 500.0mL. Mix thoroughly.
Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 45°–50°C.
Preparation of Medium:  Aseptically combine 500.0mL sterile,
cooled GC agar base solution and 500.0mL cooled sterile hemoglobin
solution. Aseptically add 10.0mL of sterile IsoVitaleX ® enrichment.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the isolation and cultivation of fastidious microorganisms,
especially Neisseria and Haemophilus species, from a variety of clini-
cal specimens.