Chocolate Agar Base with Hemoglobin and Yeast Autolysate

[所属分类:培养基配方] [发布时间:2020-4-21] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chocolate Agar Base
with Hemoglobin and Yeast Autolysate

培养基配方
Composition per liter:
Proteose peptone..............................................20.0g
Agar .........................................................15.0g
Na2HPO4 .......................................................5.0g
NaCl...........................................................5.0g
Glucose .......................................................0.5g
Hemoglobin solution.........................................500.0mL
Yeast autolysate solution....................................20.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Hemoglobin Solution:
Composition per 500.0mL:
Bovine hemoglobin.............................................10.0g
Preparation of Hemoglobin Solution: Add bovine hemoglobin to
distilled/deionized water and bring volume to 500.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Yeast Autolysate Solution:
Composition per 20.0mL:
Yeast autolysate..............................................10.0g
Glucose .......................................................1.0g
NaHCO3 .......................................................0.15g
Preparation of Yeast Autolysate Solution: Add components to
distilled/deionized water and bring volume to 20.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except hemoglobin and
yeast autolysate solutions, to distilled/deionized water and bring vol-
ume to 480.0mL. Mix thoroughly. Gently heat until boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Add 500.0mL
sterile hemoglobin solution and 20.0mL sterile yeast autolysate solu-
tion. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the isolation of Neisseria gonorrhoeae from chronic and
acute cases of gonococcal infections.