Chocolate Agar Base with Hemoglobin and Vitamino Growth Supplement

[所属分类:培养基配方] [发布时间:2020-4-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chocolate Agar Base
with Hemoglobin and Vitamino Growth Supplement

培养基配方
Composition per liter:
Proteose peptone...................................................20.0g
Agar ..............................................................15.0g
Na2HPO4 ............................................................5.0g
NaCl................................................................5.0g
Glucose ............................................................0.5g
Hemoglobin solution..............................................500.0mL
Vitamino growth supplement solution...............................10.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Hemoglobin Solution:
Composition per 500.0mL:
Bovine hemoglobin..................................................10.0g
Preparation of Hemoglobin Solution: Add bovine hemoglobin to
distilled/deionized water and bring volume to 500.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Vitamino Growth Supplement Solution:
Composition per 10.0mL:
L -Glutamine........................................................0.2g
Adenine sulfate...................................................20.0mg
Guanine hydrochlroide .............................................0.6mg
p-Aminobenzoic acid (PABA)........................................0.26mg
Vitamin B 12 ......................................................0.2mg
Preparation of Vitamino Growth Supplement Solution: Add
components to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except hemoglobin and
Vitamino growth supplement solutions, to distilled/deionized water
and bring volume to 480.0mL. Mix thoroughly. Gently heat until boil-
ing. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Add 500.0mL sterile hemoglobin solution and 10.0mL sterile Vitami-
no growth supplement solution. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the isolation of Neisseria gonorrhoeae from chronic and
acute cases of gonococcal infections.