Chocolate II Agar

[所属分类:培养基配方] [发布时间:2020-4-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chocolate II Agar

培养基配方
Composition per liter:
Agar.......................................................12.0g
Casein enzymic hydrolysate..................................7.5g
Meat extract................................................7.5g
NaCl........................................................5.0g
K2HPO4 .....................................................4.0g
Corn starch.................................................1.0g
KH2PO4 .....................................................1.0g
Vitamin B 12 ..............................................0.2mg
Hemoglobin solution .....................................500.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Hemoglobin Solution:
Composition per 500.0mL:
Bovine hemoglobin.........................................10.0g
Preparation of Hemoglobin Solution: Add bovine hemoglobin to
distilled/deionized water and bring volume to 500.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Preparation of Medium: Add components, except hemoglobin so-
lution, to distilled/deionized water and bring volume to 500.0mL. Mix
thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Add 500.0mL sterile hemoglobin
solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the isolation of Neisseria and Haemophilus species from a
variety of clinical specimens.