Chocolate No. 2 Agar Base with Supplements

[所属分类:培养基配方] [发布时间:2020-4-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chocolate No.2 Agar Base with Supplements

培养基配方
Composition per liter:
Agar...........................................................12.0g
Casein enzymic hydrolysate......................................7.5g
Meat extract ...................................................7.5g
NaCl............................................................5.0g
K2HPO4 .........................................................4.0g
Corn starch.....................................................1.0g
KH2PO4 .........................................................1.0g
Hemoglobin solution..........................................480.0mL
Supplement solution ..........................................40.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Hemoglobin Solution:
Composition per 500.0mL:
Hemoglobin ...................................................10.0g
Preparation of Hemoglobin Solution: Add hemoglobin to dis-
tilled/deionized water and bring volume to 500.0mL. Mix thoroughly.
Filter sterilize.
Supplement Solution:
Composition per 40.0mL:
p-Aminobenzoic acid........................................259.0mg
L -Glutamine...............................................100.0mg
Adenine sulfate.............................................10.0mg
NAD..........................................................2.5mg
Vitamin B 12 ................................................1.0mg
Cocarboxylase................................................1.0mg
Guanine·HCl.................................................0.3mg
Fe(NO3)3 ....................................................0.2mg
L- Cysteine·HCl............................................0.13mg
Thiamine·HCl ..............................................0.03mg
Preparation of Supplement Solution:  Add components to dis-
tilled/deionized water and bring volume to 40.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except hemoglobin so-
lution and supplement solution, to distilled/deionized water and bring
volume to 480.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°C. Aseptically add hemoglobin and sup-
plement solutions. Mix thoroughly. Pour into Petri dishes or aseptically
distribute into sterile tubes.
Use: For the isolation of Neisseria spp. and Haemophilus spp. from a
variety of clinical specimens.