Chocolate II Agar with Hemoglobin and IsoVitaleX ® (GCII Agar with Hemoglobin and IsoVitaleX ® )

[所属分类:培养基配方] [发布时间:2020-4-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chocolate II Agar with Hemoglobin and IsoVitaleX ®
(GCII Agar with Hemoglobin and IsoVitaleX ® )

培养基配方
Composition per liter:
GCII agar base..............................................990.0mL
IsoVitaleX ® enrichment......................................10.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium is available as a prepared medium from BD Di-
agnostic Systems.
GCII Agar Base:
Composition per liter:
Agar...........................................................12.0g
Hemoglobin.....................................................10.0g
Pancreatic digest of casein.....................................7.5g
Selected meat peptone ..........................................7.5g
NaCl............................................................5.0g
K2HPO4 .........................................................4.0g
Cornstarch......................................................1.0g
KH2PO4 .........................................................1.0g
Preparation of GCII Agar Base:  Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Gently heat
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C.
IsoVitaleX ® Enrichment:
Composition per liter:
Glucose.........................................................100.0g
L- Cysteine·HCl.................................................25.9g
L- Glutamine ....................................................10.0g
L- Cystine........................................................1.1g
Adenine...........................................................1.0g
Nicotinamide adenine dinucleotide ...............................0.25g
Vitamin B 12 .....................................................0.1g
Thiamine pyrophosphate ...........................................0.1g
Guanine·HCl.....................................................0.03g
Fe(NO3)3 ·6H2O..................................................0.02g
p-Aminobenzoic acid.............................................0.013g
Thiamine·HCl....................................................3.0mg
Preparation of IsoVitaleX ® : Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.
Preparation of Medium:  Aseptically add 10.0mL of sterile IsoVi-
taleX ® enrichment to 990.0L of sterile, cooled GCII agar base. Mix
thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of fastidious microorganisms,
especially Neisseria and Haemophilus species, from a variety of clini-
cal specimens.