Cholic Acid Medium

[所属分类:培养基配方] [发布时间:2020-4-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cholic Acid Medium

培养基配方
Composition per liter:
Noble agar......................................................15.0g
K2HPO4 ..........................................................3.5g
Cholic acid......................................................2.0g
(NH4)2SO4 .......................................................2.0g
KH2PO4 ..........................................................1.5g
MgSO4 ·7H2O.....................................................0.1g
CaCl2 ·2H2O ...................................................0.01g
FeSO4 ·7H2O....................................................0.5mg
pH 7.0 ± 0.2 at 25°C
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use:  For the cultivation and maintenance of Nocardia species and
other bacteria that can utilize cholic acid as a carbon source.