Choline Assay Medium

[所属分类:培养基配方] [发布时间:2020-4-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Choline Assay Medium

培养基配方
Composition per liter:
Sucrose..........................................................40.0g
Potassium sodium tartrate........................................11.4g
(NH4)2NO3 ........................................................2.0g
KH2PO4 ...........................................................2.0g
MgSO4 ·7H2O......................................................1.0g
CaCl2 ·2H2O .....................................................0.2g
NaCl..............................................................0.2g
ZnSO4 ·7H2O.....................................................0.02g
FeSO4 ...........................................................1.1mg
Na3BO3 ..........................................................0.7mg
(NH4)2MoO3 ......................................................0.5mg
CuCl.............................................................0.3mg
MgSO4 ·7H2O....................................................0.11mg
Biotin..........................................................0.01mg
pH5.5 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Continue boiling for 2–3 min. Allow precipitate to settle out.
Distribute supernatant into 125.0mL flasks in 10.0mL volumes. Add
standard solution or test solutions to each flask. Adjust the volume of
each flask to 20.0mL with distilled/deionized water. Autoclave for 10
min at 15 psi pressure–121°C.
Use: For the microbiological assay of choline using Neurospora
crassa as the test microorganism.