Chopped Liver Broth

[所属分类:培养基配方] [发布时间:2020-4-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chopped Liver Broth

培养基配方
Composition per liter:
Fresh beef liver ................................................500.0g
Peptone...........................................................10.0g
K2HPO4 ............................................................1.0g
Soluble starch.....................................................1.0g
pH 7.0 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Grind fresh beef liver. Add to 1.0L of dis-
tilled/deionized water. Gently heat and bring to boiling. Continue boil-
ing for 60 min. Cool to 25°C. Adjust pH to 7.0. Gently heat and bring
to boiling. Continue boiling for 10 min. Filter through cheesecloth.
Save chopped liver particles. To filtrate, add remaining components.
Bring volume to 1.0L with distilled/deionized water. Adjust pH to 7.0.
Filter through Whatman #1 filter paper. Add chopped liver particles to
test tubes to a depth of 1.2–2.5 cm. Add 10.0mL of broth to each tube.
Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the isolation and cultivation of Clostridium botulinum,
Clostridium perfringens, and other anaerobic bacteria from foods.