Chopped Meat Broth

[所属分类:培养基配方] [发布时间:2020-4-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chopped Meat Broth

培养基配方
Composition per liter:
Ground meat, fat free.......................................500.0g
Pancreatic digest of casein..................................30.0g
K2HPO4 .......................................................5.0g
Yeast extract.................................................5.0g
L -Cysteine·HCl .............................................0.5g
Resazurin....................................................1.0mg
NaOH (1N solution)..........................................25.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Use lean beef or horse meat. Remove fat
and connective tissue. Grind finely. Add ground meat and 25.0mL of
NaOH solution to distilled/deionized water and bring volume to
1025.0mL. Gently heat and bring to boiling. Continue boiling for 15
min without stirring. Cool to room temperature. Remove fat from sur-
face. Filter and retain both meat particles and filtrate. Adjust volume of
filtrate to 1.0L with distilled/deionized water. Add pancreatic digest of
casein, K2 HPO4 , yeast extract, and resazurin. Gently heat and bring to
boiling. Boil for 1–2 min. Add  L -cysteine·HCl. Mix thoroughly. Dis-
tribute 7.0mL into tubes that contain meat particles (1 part meat parti-
cles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure–121°C.
Use: For the cultivation of various anaerobes.