Chopped Meat Broth with Carbohydrates (DSMZ Medium 110)

[所属分类:培养基配方] [发布时间:2020-4-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chopped Meat Broth with Carbohydrates
(DSMZ Medium 110)

培养基配方
Composition per liter:
Ground meat, fat free........................................500.0g
Pancreatic digest of casein...................................30.0g
K2HPO4 ........................................................5.0g
Yeast extract..................................................5.0g
Glucose .......................................................4.0g
Cellobiose ....................................................1.0g
Maltose........................................................1.0g
Starch, soluble................................................1.0g
L -Cysteine·HCl...............................................0.5g
Resazurin ....................................................1.0mg
NaOH (1N solution)...........................................25.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Use lean beef or horse meat. Remove fat
and connective tissue. Grind finely. Add ground meat and 25.0mL of
NaOH solution to distilled/deionized water and bring volume to
1025.0mL. Gently heat and bring to boiling. Continue boiling for 15
min without stirring. Cool to room temperature. Remove fat from sur-
face. Filter and retain both meat particles and filtrate. Adjust volume of
filtrate to 1.0L with distilled/deionized water. Add pancreatic digest of
casein, K2HPO4 , yeast extract, and resazurin. Gently heat and bring to
boiling. Boil for 1–2 min. Add glucose, cellobiose, maltose, and solu-
ble starch. Add  L -cysteine·HCl. Mix thoroughly. Distribute 7.0mL into
tubes that contain meat particles (1 part meat particles to 5 parts fluid).
Autoclave for 30 min at 15 psi pressure–121°C.
Use: For the cultivation of numerous anaerobes, including Actinomy-
ces suis, Anaerobiospirillum succiniciproducens, Bacteroides distaso-
nis, Bacteroides eggerthii, Bacteroides fragilis, Bacteroides helco-
genes, Bacteroides macacae, Bacteroides pyogenes, Bacteroides
splanchnicus, Bacteroides suis, Centipeda periodontii, numerous
Clostridium species, Eubacterium brachy, Eubacterium eligens,
Eubacterium hallii, Eubacterium limosum, Eubacterium plautii,
Eubacterium ruminantium, Eubacterium saburreum, Eubacterium sir-
aeum, Eubacterium species, Eubacterium tarantellus, Eubacterium
tenue, Eubacterium ventriosum, Megamonas hypermegas, Peptococ-
cus niger, Peptostreptococcus productus, Prevotella buccae, Pre-
votella buccalis, Prevotella denticola, Prevotella intermedia, Pre-
votella oralis, and Selenomonas sputigena.