Chopped Meat Broth with Formate and Fumarate (LMG Medium 69)

[所属分类:培养基配方] [发布时间:2020-4-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chopped Meat Broth with Formate and Fumarate
(LMG Medium 69)

培养基配方
Composition per liter:
Ground meat, fat free........................................500.0g
Pancreatic digest of casein...................................30.0g
K2HPO4 ........................................................5.0g
Yeast extract..................................................5.0g
L -Cysteine·HCl...............................................0.5g
Resazurin ....................................................1.0mg
NaOH (1N solution)...........................................25.0mL
Formate-fumarate solution.....................................7.7mL
pH 7.0 ± 0.2 at 25°C
Formate-Fumarate Solution:
Composition per 100.0mL:
Sodium formate ................................................6.0g
Fumaric acid ..................................................6.0g
Preparation of Formate-Fumarate Solution: Add components
to distilled/deionized water and bring volume to 100.0mL. Adjust pH
to 7.0. Filter sterilize.
Preparation of Medium: Use lean beef or horse meat. Remove fat
and connective tissue. Grind finely. Add ground meat and 25.0mL of
NaOH solution to distilled/deionized water and bring volume to
1025.0mL. Gently heat and bring to boiling. Continue boiling for 15
min without stirring. Cool to room temperature. Remove fat from sur-
face. Filter and retain both meat particles and filtrate. Adjust volume of
filtrate to 1.0L with distilled/deionized water. Add pancreatic digest of
casein, agar, K2HPO4 , yeast extract, and resazurin. Gently heat and
bring to boiling. Boil for 1–2 min. Add  L -cysteine·HCl. Mix thorough-
ly. Distribute 6.5mL into tubes that contain meat particles (1 part meat
particles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure–
121°C. Cool to 25°C. Aseptically add 50 μL of sterile formate/fumar-
ate solution to each tube prior to inoculation.
Use: For the cultivation of various Clostridium spp.