Chopped Meat Broth with Vitamin K 1 (LMG Medium 70)

[所属分类:培养基配方] [发布时间:2020-4-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chopped Meat Broth with Vitamin K 1
(LMG Medium 70)

培养基配方
Composition per liter:
Ground meat, fat free..........................................500.0g
Pancreatic digest of casein.....................................30.0g
K2HPO4 ..........................................................5.0g
Yeast extract....................................................5.0g
L -Cysteine·HCl ................................................0.5g
Resazurin ......................................................1.0mg
NaOH(1N solution)..............................................25.0mL
Vitamin K 1 solution............................................7.7mL
pH 7.0 ± 0.2 at 25°C
Vitamin K 1 Solution:
Composition per 50.0mL:
Ethanol (20% solution).........................................50.0mL
Vitamin K 1 ....................................................0.7gL
Preparation of Vitamin K 1 Solution: Mix components. Filter
sterilize. Store solution protected from light at 5°C. Discard after one
month.
Preparation of Medium: Use lean beef or horse meat. Remove fat
and connective tissue. Grind finely. Add ground meat and 25.0mL of
NaOH solution to distilled/deionized water and bring volume to
1025.0mL. Gently heat and bring to boiling. Continue boiling for 15
min without stirring. Cool to room temperature. Remove fat from sur-
face. Filter and retain both meat particles and filtrate. Adjust volume of
filtrate to 1.0L with distilled/deionized water. Add pancreatic digest of
casein, agar, K2HPO4 , yeast extract, and resazurin. Gently heat and
bring to boiling. Boil for 1–2 min. Add  L -cysteine·HCl. Mix thorough-
ly. Distribute 6.5mL into tubes that contain meat particles (1 part meat
particles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure–
121°C. Cool to 25°C. Aseptically add 50 μL of sterile vitamin K 1  solu-
tion to each tube prior to inoculation.
Use: For the cultivation of various Clostridium spp.