Christensen’s Urea Agar

[所属分类:培养基配方] [发布时间:2020-4-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Christensen’s Urea Agar

培养基配方
Composition per liter:
Agar............................................................................15.0g
NaCl.............................................................................5.0g
KH2PO4 ..........................................................................2.0g
Peptone .........................................................................1.0g
Glucose..........................................................................1.0g
Phenol Red.....................................................................0.012g
Urea solution.................................................................100.0mL
pH 6.8 ± 0.1 at 25°C
Urea Solution:
Composition per 100.0mL :
Urea...........................................................................20.0g
Preparation of Urea: Add urea to 100.0mL of distilled/deionized wa-
ter. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except urea solution, to
distilled/deionized water and bring volume to 900.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50–55°C. Aseptically add 100.0mL of sterile
urea solution. Mix thoroughly. Pour into Petri dishes or distribute into
sterile tubes. Allow tubes to solidify in a slanted position.
Use:  For the differentiation of a variety of microorganisms, especially
members of the Enterobacteriaceae, aerobic actinomycetes, strepto-
cocci, and nonfermenting Gram-negative bacteria, on the basis of ure-
ase production.