Christensen’s Urea Agar with Sodium Chloride (BAM M40)

[所属分类:培养基配方] [发布时间:2020-4-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Christensen’s Urea Agar with Sodium Chloride
(BAM M40)

培养基配方
Composition per liter:
NaCl............................................................................20.0g
Agar............................................................................15.0g
KH2PO4 ..........................................................................2.0g
Peptone .........................................................................1.0g
Glucose..........................................................................1.0g
Phenol Red.....................................................................0.012g
Urea solution.................................................................100.0mL
pH 6.8 ± 0.1 at 25°C
Urea Solution:
Composition per 100.0mL :
Urea............................................................................20.0g
Preparation of Urea:  Add urea to 100.0mL of distilled/deionized
water. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except urea solution, to
distilled/deionized water and bring volume to 900.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50–55°C. Aseptically add 100.0mL of sterile
urea solution. Mix thoroughly. Pour into Petri dishes or distribute into
sterile tubes. Allow tubes to solidify in a slanted position.
Use:For the differentiation of halophilic Vibrio spp. on the basis of
urease production.