Clostridium acetobutylicum Medium

[所属分类:培养基配方] [发布时间:2020-5-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium acetobutylicum Medium

培养基配方
Composition per liter:
Potato flakes, dried ....................................................40.0g
Glucose...................................................................6.0g
CaCO3 ....................................................................2.0g
L -Cysteine·HCl·H2O.....................................................0.5g
Resazurin................................................................1.0mg
Preparation of Medium: Add potato flakes and CaCO3 to distilled/
deionized water and bring volume to 1.0L. Autoclave for 30 min at 15
psi pressure–121°C. Filter through cheesecloth. To filtrate, add glu-
cose,  L -cysteine·HCl·H2O, and resazurin. Autoclave for 20 min at 15
psi pressure–121°C. Distribute into sterile tubes or flasks.
Use:  For the growth and maintenance of Clostridium acetobutylicum,
Clostridium beijerinckii, Clostridium butyricum, Clostridium roseum,
and other Clostridium species.