Clostridium acidurici Medium

[所属分类:培养基配方] [发布时间:2020-5-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium acidurici Medium

培养基配方
Composition per liter:
Uric acid....................................................................2.0g
Yeast extract................................................................1.0g
K2HPO4 .....................................................................0.91g
KOH.........................................................................0.67g
Sodium thioglycolate.........................................................0.5g
MgSO4 ·7H2O................................................................0.25g
CaCl2 ·2H2O...............................................................0.015g
FeSO4 ·7H2O................................................................6.0mg
NaHCO3  solution ..........................................................25.0mL
Sodium thioglycolate solution..............................................25.0mL
pH 7.0–7.5 at 25°C
NaHCO3 Solution:
Composition per 25.0mL:
NaHCO3 ......................................................................5.0g
Preparation of NaHCO3 Solution: Add NaHCO3  to distilled/de-
ionized water and bring volume to 25.0mL. Mix thoroughly. Filter ster-
ilize.
Sodium Thioglycolate Solution:
Composition per 25.0mL:
Sodium thioglycolate.........................................................0.5g
Preparation of Sodium Thioglycolate Solution: Add sodium
thioglycolate to distilled/deionized water and bring volume to 25.0mL.
Mix thoroughly. Autoclave solution separately for 15 min at 15 psi
pressure–121°C.
Preparation of Medium: Add K2HPO4 and KOH to distilled/deion-
ized water and bring volume to 1.0L. Add uric acid. Gently heat until boil-
ing. Add the remaining components, except NaHCO3 and sodium
thioglycolate. Mix thoroughly. Adjust pH to 7.0–7.5. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Add 0.25mL of
sterile NaHCO3  solution and 0.25mL of sterile sodium thioglycolate solu-
tion for each 10.0mL of sterile basal medium.
Use:  For the cultivation and maintenance of Clostridium acidurici,
Clostridium purinolyticum, and other bacteria that can utilize uric acid
as a carbon source.