​Clostridium aldrichii Agar

[所属分类:培养基配方] [发布时间:2020-5-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium aldrichii Agar

培养基配方
Composition per liter:
Agar .....................................................................15.0g
Cellobiose ...............................................................10.0g
NH4Cl .....................................................................2.0g
K2HPO4 ...................................................................1.65g
NaCl.......................................................................0.9g
(NH4)2SO4 .................................................................0.9g
L -Cysteine·HCl·H2O......................................................0.5g
KCl........................................................................0.5g
MgSO4 ·7H2O...............................................................0.5g
Trypticase™................................................................0.5g
Yeast extract..............................................................0.5g
Na2S·9H2O.................................................................0.2g
CaCl2 ....................................................................0.09g
Resazurin ................................................................0.5mg
Wolfe’s mineral solution................................................10.0mL
Wolfe’s vitamin solution................................................10.0mL
NaHCO3 ................................................................variable
pH 7.0 ± 0.2 at 25°C
Wolfe’s Mineral Solution:
Composition per liter:
MgSO4 ·7H2O..............................................................3.0g
Nitrilotriacetic acid.....................................................1.5g
NaCl......................................................................1.0g
MnSO4 ·H2O...............................................................0.5g
CaCl2 ....................................................................0.1g
CoCl2 ·6H2O..............................................................0.1g
FeSO4 ·7H2O..............................................................0.1g
ZnSO4 ·7H2O..............................................................0.1g
AlK(SO4)2 ·12H2O........................................................0.01g
CuSO4 ·5H2O.............................................................0.01g
H3BO3 ...................................................................0.01g
Na2MoO4 ·2H2O...........................................................0.01g
Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic
acid to approximately 500.0mL of water and adjust to pH 6.5 with
KOH to dissolve the compound. Bring volume to 1.0L with remaining
water and add remaining compounds one at a time.
Wolfe’s Vitamin Solution:
Composition per liter:
Pyridoxine·HCl..........................................................10.0mg
p-Aminobenzoic acid.......................................................5.0mg
Lipoic acid...............................................................5.0mg
Nicotinic acid............................................................5.0mg
Riboflavin................................................................5.0mg
Thiamine·HCl ............................................................5.0mg
Calcium  DL -pantothenate.................................................5.0mg
Biotin....................................................................2.0mg
Folic acid ...............................................................2.0mg
Vitamin B 12 .............................................................0.1mg
Preparation of Wolfe’s Vitamin Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Prepare and dispense medium under 80%
N2 + 20% CO2 . Add components, except NaHCO3 , to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Gently heat and
bring to boiling. Continue boiling for 3 min. Cool to room temperature
while sparging with 80% N2 + 20% CO2 . Add sufficient NaHCO3  to
bring the pH to 7.0. Distribute into tubes or flasks. Autoclave for 15
min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in
tubes.
Use: For the cultivation of Clostridium aldrichii.