Clostridium aminobutyricum Medium

[所属分类:培养基配方] [发布时间:2020-5-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium aminobutyricum Medium

培养基配方
Composition per liter:
K2HPO4 .....................................................................7.05g
Yeast extract................................................................3.0g
KH2PO4 .....................................................................1.29g
MgCl2 ·6H2O.................................................................0.2g
CaCl2 ·2H2O................................................................0.01g
FeCl3 ·6H2O................................................................0.01g
Methylene Blue..............................................................2.0mg
MnSO4 ·H2O.................................................................1.0mg
Na2MoO4 ·2H2O .............................................................1.0mg
γ-Aminobutyrate solution ................................................100.0mL
Na2CO3 solution...........................................................100.0mL
Na2S·9H2O solution........................................................50.0mL
pH 7.4–7.7 at 25°C
γ-Aminobutyrate Solution:
Composition per 100.0mL:
γ-Aminobutyrate.............................................................5.0g
Preparation of γ-Aminobutyrate Solution: Add γ-aminobu-
tyrate to distilled/deionized water and bring volume to 100.0mL. Mix
thoroughly. Filter sterilize.
Na2CO3 Solution:
Composition per 100.0mL:
Na2CO3 .....................................................................2.0g
Preparation of Na2CO3 Solution: Add Na2CO3 to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C.
Na2S·9H2O Solution:
Composition per 50.0mL:
Na2S·9H2O...................................................................0.3g
Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 50.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except γ-aminobu-
tyrate solution, Na2CO3 solution, and Na2S·9H2O solution, to distilled/
deionized water and bring volume to 750.0mL. Autoclave for 15 min
at 15 psi pressure–121°C. Cool under 80% N2 + 10% CO2 + 10% H2 .
Aseptically add the sterile γ-aminobutyrate solution, Na2CO3 solution,
and Na2S·9H2O solution. Adjust pH to 7.4–7.7. Distribute using anaer-
obic technique into tubes or flasks.
Use:  For the cultivation and maintenance of Clostridium aminobutyri-
cum and other bacteria which can utilize aminobutyric acid as a carbon
source.