Clostridium aminobutyricum Medium

[所属分类:培养基配方] [发布时间:2020-5-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium aminobutyricum Medium

培养基配方
Composition per liter:
K2HPO4 ........................................................................7.0g
γ-Aminobutyric acid...........................................................5.0g
Yeast extract..................................................................3.0g
Agar...........................................................................1.5g
KH2PO4 ........................................................................1.3g
MgCl2 ·6H2O ..................................................................0.2g
CaCl2 ·2H2O .................................................................0.01g
FeCl3 ·6H2O..................................................................0.01g
Na2MoO4 ·2H2O................................................................1.0mg
Resazurin ....................................................................1.0mg
NaHCO3  solution.............................................................20.0mL
Na2S·9H2O solution...........................................................20.0mL
pH 7.2 ± 0.2 at 25°C
NaHCO3 Solution:
Composition per 20.0mL:
NaHCO3 ........................................................................1.0g
Preparation of NaHCO3 Solution:  Add NaHCO3 to distilled/de-
ionized water and bring volume to 20.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C.
Na2S·9H2O Solution:
Composition per 20.0mL:
Na2S·9H2O.....................................................................0.3g
Preparation of Na2S·9H2O Solution:  Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 20.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except NaHCO3  solu-
tion and Na2S·9H2O solution, to distilled/deionized water and bring
volume to 960.0mL. Mix thoroughly. Sparge under 80% N2 + 20%
CO2.  Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and
anaerobically add 20.0mL of sterile NaHCO3  solution and 20.0mL of
sterile Na2S·9H2O solution. Mix thoroughly.
Use:  For the growth and maintenance of Clostridium aminobutyricum
and other Clostridium species.