Clostridium aminovalericum Medium

[所属分类:培养基配方] [发布时间:2020-5-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium aminovalericum Medium

培养基配方
Composition per liter:
K2HPO4 ................................................................9.77g
5-Aminovaleric acid ....................................................6.0g
Yeast extract...........................................................5.0g
Mannitol................................................................1.0g
KH2PO4 ................................................................0.54g
Sodium thioglycolate....................................................0.5g
MgSO4 .................................................................0.06g
CaSO4 ·2H2O..........................................................0.034g
Trace elements solution SL-6 ..........................................1.0mL
pH 7.9 ± 0.2 at 25°C
Trace Elements Solution SL-6:
Composition per liter:
H3BO3 ..................................................................0.3g
CoCl2 ·6H2O ...........................................................0.2g
ZnSO4 ·7H2O............................................................0.1g
MnCl2 ·4H2O...........................................................0.03g
Na2MoO4 ·H2O .........................................................0.03g
NiCl2 ·6H2O...........................................................0.02g
CuCl2 ·2H2O ..........................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.4.
Preparation of Medium: Prepare medium under 100% N2 . Add
components to distilled/deionized water and bring volume to 1.0L. Au-
toclave for 15 min at 15 psi pressure–121°C. Adjust pH to 7.9. Distrib-
ute into tubes or flasks under 100% N2 .
Use: For the cultivation and maintenance of Clostridium aminovaler-
icum and other bacteria that can utilize aminovaleric acid as a carbon
source.