Clostridium beijerinckii Agar

[所属分类:培养基配方] [发布时间:2020-5-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium beijerinckii Agar

培养基配方
Composition per liter:
Agar ...................................................................20.0g
K2HPO4 ..................................................................5.0g
Proteose peptone No.3....................................................5.0g
Sodium thioglycolate.....................................................5.0g
Yeast extract............................................................5.0g
Polygalacturonic acid solution.........................................50.0mL
pH 7.5 ± 0.2 at 25°C
Polygalacturonic Acid Solution:
Composition per liter:
Polygalacturonic acid (or pectin)........................................4.6g
Preparation of Polygalacturonic Acid Solution: Dissolve po-
lygalacturonic acid or pectin in small amounts of distilled/deionized
water neutralized with 10% NaOH to pH 7.2. Mix intensively. Bring
volume to 50.0mL with distilled/deionized water.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.5.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes.
Use:  For the cultivation and maintenance of Clostridium beijerinckii.