Clostridium thermocellum Medium

[所属分类:培养基配方] [发布时间:2020-5-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium thermocellum Medium

培养基配方
Composition per liter:
Cellulose .....................................................................10.0g
H2HPO4 ·3H2O...................................................................7.2g
Sodium-β-glycerophosphate......................................................6.0g
Yeast extract...................................................................4.5g
MgCl2 ·6H2O ...................................................................2.6g
KH 2 PO 4 .....................................................................1.43g
(NH 4 ) 2 SO 4 .................................................................1.3g
Glutathione....................................................................0.25g
CaCl2 ·2H2O...................................................................0.13g
FeSO4 ·7H2O ..................................................................1.1mg
Resazurin .....................................................................1.0mg
pH 7.0–7.2 at 25°
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0–7.2.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation of Clostridium thermocellum.