Clostridium thermocellum Medium

[所属分类:培养基配方] [发布时间:2020-5-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium thermocellum Medium

培养基配方
Composition per liter:
H2HPO4 ·3H2O............................................................7.2g
Sodium-β-glycerophosphate...............................................6.0g
Cellobiose ..............................................................5.0g
Yeast extract............................................................4.5g
MgCl2 ·6H2O ............................................................2.6g
KH2PO4 .................................................................1.43g
(NH4)2SO4 ...............................................................1.3g
Glutathione.............................................................0.25g
CaCl2 ·2H2O............................................................0.13g
FeSO4 ·7H2O ...........................................................1.1mg
Resazurin ..............................................................1.0mg
pH 7.0–7.2 at 25°C
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0–7.2.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation of Clostridium thermocellum.