Clostridium thermocellum Medium

[所属分类:培养基配方] [发布时间:2020-5-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium thermocellum Medium

培养基配方
Composition per liter:
Agar ....................................................................30.0g
Morpholinopropane sulfonic acid..........................................10.0g
Yeast extract.............................................................6.0g
Urea......................................................................2.0g
KH2PO4 ...................................................................1.0g
K2HPO4 ...................................................................1.0g
MgCl2 ·6H2O .............................................................0.5g
CaCl2 ·2H2O ............................................................0.05g
FeSO4 ·7H2O............................................................1.25mg
Resazurin ...............................................................1.0mg
Glucose solution .......................................................50,0mL
L -Cysteine·HCl solution ..............................................20.0mL
pH 7.2 ± 0.2 at 25°C
Glucose Solution:
Composition per 50.0mL:
D- Glucose...............................................................5.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 50.0mL. Mix thoroughly. Filter steril-
ize. Sparge with 100% N2 gas. Warm to 50°–55°C.
L -Cysteine·HCl Solution:
Composition per 10.0mL:
L -Cysteine·HCl ........................................................0.3g
Preparation of  L -Cysteine·HCl Solution : Add  L -cysteine·HCl to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize. Sparge with 100% N2 gas. Warm to 50°–55°C.
Preparation of Medium: Prepare and dispense medium under
100% N2 . Add components, except glucose solution and  L -
cysteine·HCl solution, to distilled/deionized water and bring volume to
930.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically
and anaerobically add 50.0mL of sterile glucose solution and 20.0mL
of sterile  L -cysteine·HCl solution. Mix thoroughly. Pour into sterile Pe-
tri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Clostridium thermocel-
lum.