CM4 Medium

[所属分类:培养基配方] [发布时间:2020-5-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CM4 Medium

培养基配方
Composition per liter:
Cellobiose......................................................................6.0g
Yeast extract...................................................................5.0g
K2HPO4 .........................................................................2.9g
KH2PO4 .........................................................................1.5g
(NH4)2SO4 ......................................................................1.3g
NaCl............................................................................1.0g
MgCl2 .........................................................................0.75g
Sodium thioglycolate............................................................0.5g
CaCl2 .......................................................................0.0132g
Resazurin (1.0% solution) .....................................................0.2mL
FeSO4 (1.25% solution).........................................................0.1mL
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat until
boiling. Boil until color changes from red to colorless, indicating a re-
duced state. Cool. Distribute into tubes or flasks under 97% N2 + 3%
H2 . Cap with rubber stoppers. Autoclave for 15 min at 15 psi pressure–
121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Clostridium species and
other bacteria that can utilize cellobiose as a carbon source.